Author: Alexander M. Price; Katharina E. Hayer; Daniel P. Depledge; Angus C. Wilson; Matthew D. Weitzman
Title: Novel splicing and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts Document date: 2019_12_13
ID: 2fcl6f4i_1
Snippet: polyadenylation sites have been characterized using low-throughput biochemical techniques or 23 short read cDNA-based sequencing, these technologies do not fully capture the complexity of the 24 adenoviral transcriptome. By combining Illumina short-read and nanopore long-read direct RNA 25 sequencing approaches, we mapped transcription start sites and cleavage and polyadenylation 26 sites across the adenovirus genome. The canonical viral early an.....
Document: polyadenylation sites have been characterized using low-throughput biochemical techniques or 23 short read cDNA-based sequencing, these technologies do not fully capture the complexity of the 24 adenoviral transcriptome. By combining Illumina short-read and nanopore long-read direct RNA 25 sequencing approaches, we mapped transcription start sites and cleavage and polyadenylation 26 sites across the adenovirus genome. The canonical viral early and late RNA cassettes were 27
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