Selected article for: "read sequencing and RNA seq"
Author: Alexander M. Price; Katharina E. Hayer; Daniel P. Depledge; Angus C. Wilson; Matthew D. Weitzman
Title: Novel splicing and open reading frames revealed by long-read direct RNA sequencing of adenovirus transcripts
  • Document date: 2019_12_13
    • ID: 2fcl6f4i_9
    Snippet: In this study, we have re-annotated the Ad5 genome and transcriptome using a 123 combination of short-read and long-read RNA sequencing technologies. The high read depth and 124 accuracy of base-calling achieved by Illumina-based short-read sequencing allowed for both the 125 detection of single nucleotide polymorphisms within transcriptionally active regions of the viral 126 DNA genome, as well as error-correction of the inherently noisier base-.....
    Document: In this study, we have re-annotated the Ad5 genome and transcriptome using a 123 combination of short-read and long-read RNA sequencing technologies. The high read depth and 124 accuracy of base-calling achieved by Illumina-based short-read sequencing allowed for both the 125 detection of single nucleotide polymorphisms within transcriptionally active regions of the viral 126 DNA genome, as well as error-correction of the inherently noisier base-calling of Nanopore-based 127 long-read direct RNA sequencing (dRNA-seq). dRNA-seq enabled the detection of full-length 128 RNA transcripts and the assignment of TSS and CPAS transcriptome-wide. Furthermore, by 129 combining highly accurate splice site junctions from short-read sequencing and full-length isoform 130 context from long-read sequencing, we were able to reevaluate the splicing complexity of AdV The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2019.12.13.876037 doi: bioRxiv preprint

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