Selected article for: "primer probe and probe primer"
Author: Dupas Enora; Briand Martial; Jacques Marie-Agnès; Cesbron Sophie
Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues
  • Document date: 2019_7_11
    • ID: 65qpcsiq_19
    Snippet: The tetraplex qPCR assays designed in this study were optimized for: i) primer and probe hybridization 213 temperature that was checked individually by PCR using a gradient ranging from 57.5 to 61. ng.µl -1 of BSA (ThermoFisher) and 1 µL of extracted DNA. The optimal thermocycling conditions 226 selected were: 3 min at 95°C, followed by 40 cycles of 15 s at 95°C and 30 s at 60°C. The qPCR assays 227 results were analyzed, with expert verific.....
    Document: The tetraplex qPCR assays designed in this study were optimized for: i) primer and probe hybridization 213 temperature that was checked individually by PCR using a gradient ranging from 57.5 to 61. ng.µl -1 of BSA (ThermoFisher) and 1 µL of extracted DNA. The optimal thermocycling conditions 226 selected were: 3 min at 95°C, followed by 40 cycles of 15 s at 95°C and 30 s at 60°C. The qPCR assays 227 results were analyzed, with expert verification, using Bio-Rad CFX Manager 3.1 software and its 228 regression mode. The reaction efficiency was calculated using serial dilutions with the formula: E = 229 10 (-1/slope) . 230

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