Author: Stefania Marzinotto; Catia Mio; Adriana Cifu'; Roberto Verardo; Corrado Pipan; Claudio Schneider; Francesco Curcio
Title: A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA extraction Document date: 2020_4_11
ID: mi6w8ppe_19
Snippet: Considering the performance of our pretreatment protocol in generating a higher amount of viral RNA compared to the automated one, we tested its sensitivity in assessing viral copies with the two amplification methods (RT-qPCR and RT-ddPCR). We performed a 5-fold serial dilution of the same sample used in Figure 1 and assessed amplification curves in RT-qPCR. As shown in Figure 3 , panel A, we were able to evaluate viral copies even in dilutions .....
Document: Considering the performance of our pretreatment protocol in generating a higher amount of viral RNA compared to the automated one, we tested its sensitivity in assessing viral copies with the two amplification methods (RT-qPCR and RT-ddPCR). We performed a 5-fold serial dilution of the same sample used in Figure 1 and assessed amplification curves in RT-qPCR. As shown in Figure 3 , panel A, we were able to evaluate viral copies even in dilutions as high as 1:3125. To effectively assess how many actual viral copies our protocol is able to detect, we used the same approach using RT-ddPCR. As shown in Figure 3 , panel B, our in-house protocol is able to detect as few as 10 SARS-CoV-2 copies directly from 5µL nasopharyngeal swabderived material.
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