Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_37
Snippet: The gBlock double stranded DNA surrogates of ZIKV sequences were designed to include a T7 RNA polymerase promoter at their 5'-ends. These gBlocks were cloned into the pCR2.1-TOPO vector (Fisher Scientific, Hampton, NH) by Gibson assembly (NEB) according to the manufacturer's instructions. 50 Cloned plasmids were selected and maintained in an E. coli Top10 strain. Plasmid minipreps were prepared from these strains using Qiagen miniprep kit (Qiagen.....
Document: The gBlock double stranded DNA surrogates of ZIKV sequences were designed to include a T7 RNA polymerase promoter at their 5'-ends. These gBlocks were cloned into the pCR2.1-TOPO vector (Fisher Scientific, Hampton, NH) by Gibson assembly (NEB) according to the manufacturer's instructions. 50 Cloned plasmids were selected and maintained in an E. coli Top10 strain. Plasmid minipreps were prepared from these strains using Qiagen miniprep kit (Qiagen, Valencia, CA, USA). All gBlock inserts were verified by Sanger sequencing at the Institute of Cellular and Molecular Biology Core DNA Sequencing Facility.
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