Selected article for: "host virus protein and virus host"

Author: Myra Hosmillo; Jia Lu; Michael R. McAllaster; James B. Eaglesham; Xinjie Wang; Edward Emmott; Patricia Domingues; Yasmin Chaudhry; Timothy J Fitzmaurice; Matthew K.H. Tung; Marc Panas; Gerald McInerney; Nicholas Locker; Craig B. Willen; Ian Goodfellow
Title: Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation
  • Document date: 2019_3_8
  • ID: d0q5lhf4_6
    Snippet: We have previously described a novel paradigm of viral translation that relies on the 36 interaction of host translation initiation factors with a virus-encoded protein (VPg), 37 covalently linked to the 5' end of the genome of members of the Caliciviridae family 38 of positive sense RNA viruses (Chaudhry et al., 2006; Chung et al., 2014; 39 7 positions were previously identified using a transposon based mutagenesis screen 115 as sites that toler.....
    Document: We have previously described a novel paradigm of viral translation that relies on the 36 interaction of host translation initiation factors with a virus-encoded protein (VPg), 37 covalently linked to the 5' end of the genome of members of the Caliciviridae family 38 of positive sense RNA viruses (Chaudhry et al., 2006; Chung et al., 2014; 39 7 positions were previously identified using a transposon based mutagenesis screen 115 as sites that tolerate insertions, without compromising virus viability (Thorne et al., 116 2012) . Our approach was somewhat analogous to that recently published for 117 coronaviruses (V'kovski et al., 2019) but instead used stable isotope labelling of 118 permissive cells and the FLAG affinity purification tag rather than proximity labelling. 119

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