Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_29
Snippet: In order to showcase the ability of MTK to generate libraries of transcriptional unit variants, we performed two combinatorial reactions: First, a library of fluorescent proteins that vary in their localization, and which can be integrated as a single-copy into the BxBI landing pad site; second, a library of viral-delivered sgRNAs that target Cas9 to two fluorescent proteins leading to their disruption......
Document: In order to showcase the ability of MTK to generate libraries of transcriptional unit variants, we performed two combinatorial reactions: First, a library of fluorescent proteins that vary in their localization, and which can be integrated as a single-copy into the BxBI landing pad site; second, a library of viral-delivered sgRNAs that target Cas9 to two fluorescent proteins leading to their disruption.
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