Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells Document date: 2018_12_26
ID: 1kugu5zk_53
Snippet: Forward and reverse primers ordered from IDT (www.idtdna.com) were manually designed to anneal to source DNA. Internal BsaI and BsmBI sites were removed and tandem BsaI and BsmBI sites were appended to both the 5' and 3' ends of the sequence. PCR The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint Geneblocks. Gene fragments were ordered from IDT as ei.....
Document: Forward and reverse primers ordered from IDT (www.idtdna.com) were manually designed to anneal to source DNA. Internal BsaI and BsmBI sites were removed and tandem BsaI and BsmBI sites were appended to both the 5' and 3' ends of the sequence. PCR The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint Geneblocks. Gene fragments were ordered from IDT as either whole constructs or partial constructs with complementary overhangs to ensure proper domestication.
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