Author: Deguo Wang
Title: One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay Document date: 2020_4_22
ID: krim73zt_9
Snippet: The detection limits of the one-pot real-time RT-LAMP assay and the one-pot visual RT-LAMP assay were determined using pUC57-N DNA ranging from 2-0.02 fg at 59 °C for 60 min in a StepOneTM System (Applied Biosystems, Foster City, CA, USA) or in a water bath. The detection limits of both the one-pot real-time RT-LAMP assay and the one-pot visual RT-LAMP assay were found to be ≥ 0.2 fg pUC57-N DNA (Figure 3) , which was equivalent to ≥ 6 copie.....
Document: The detection limits of the one-pot real-time RT-LAMP assay and the one-pot visual RT-LAMP assay were determined using pUC57-N DNA ranging from 2-0.02 fg at 59 °C for 60 min in a StepOneTM System (Applied Biosystems, Foster City, CA, USA) or in a water bath. The detection limits of both the one-pot real-time RT-LAMP assay and the one-pot visual RT-LAMP assay were found to be ≥ 0.2 fg pUC57-N DNA (Figure 3) , which was equivalent to ≥ 6 copies.
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