Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_12
Snippet: A limitation of this work is that DNA template was used as a positive control for the viral RNA. Even though RNaseP demonstrates comparable stability to that of the DNA control, viral RNA might be more susceptible to nucleases in the NP and OP samples. Our next step is to use an in vitro transcribed RNA as spike-in, fully realizing that naked transcripts are likely more easily degraded by the nucleases than viral RNA with the protection of protei.....
Document: A limitation of this work is that DNA template was used as a positive control for the viral RNA. Even though RNaseP demonstrates comparable stability to that of the DNA control, viral RNA might be more susceptible to nucleases in the NP and OP samples. Our next step is to use an in vitro transcribed RNA as spike-in, fully realizing that naked transcripts are likely more easily degraded by the nucleases than viral RNA with the protection of protein coating. The ultimate test is using positive patient samples containing viral particles.
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