Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_15
Snippet: Extraction control HCT-116 cells were counted and pipetted directly to the QE buffer, and no swab was used. A positive control was made for each sample by transferring 100 ul of sample to a new 1.5 mL Eppendorf tube and adding 10000 copies of COVID19 plasmid DNA template control (IDT Cat. No 10006625), prior to extraction incubation. The final concentration of the positive control is 100 copies/ul. No cell input negative control samples were also.....
Document: Extraction control HCT-116 cells were counted and pipetted directly to the QE buffer, and no swab was used. A positive control was made for each sample by transferring 100 ul of sample to a new 1.5 mL Eppendorf tube and adding 10000 copies of COVID19 plasmid DNA template control (IDT Cat. No 10006625), prior to extraction incubation. The final concentration of the positive control is 100 copies/ul. No cell input negative control samples were also used for Figure 1 and Table 2 . In Figure 1 , the no cell input negative control also had a plasmid DNA template positive control counterpart.
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