Author: Aiping Wu; Peihua Niu; Lulan Wang; Hangyu Zhou; Xiang Zhao; Wenling Wang; Jingfeng Wang; Chengyang Ji; Xiao Ding; Xianyue Wang; Roujian Lu; Sarah Gold; Saba Aliyari; Shilei Zhang; Ellee Vikram; Angela Zou; Emily Lenh; Janet Chen; Fei Ye; Na Han; Yousong Peng; Haitao Guo; Guizhen Wu; Taijiao Jiang; Wenjie Tan; Genhong Cheng
Title: Mutations, Recombination and Insertion in the Evolution of 2019-nCoV Document date: 2020_3_2
ID: jmrg4oeb_39
Snippet: Given the whole protein sequences corresponding for the 2019-nCoV, 2019-nCoVs were 5 obtained from ViPR -Coronavirus and GISAID database on 18 th Feb, 2020. We For the spike protein, given the whole genomes of 2019 nCoVs (118 strains) and other SARS-like viruses isolated from Bat(1 strains) and Pangolin (6 strains), the genes was predicted by GeneMarkS (version 3.36). The predicted genes were then against the proteome of SARS-CoV by BLAST (versio.....
Document: Given the whole protein sequences corresponding for the 2019-nCoV, 2019-nCoVs were 5 obtained from ViPR -Coronavirus and GISAID database on 18 th Feb, 2020. We For the spike protein, given the whole genomes of 2019 nCoVs (118 strains) and other SARS-like viruses isolated from Bat(1 strains) and Pangolin (6 strains), the genes was predicted by GeneMarkS (version 3.36). The predicted genes were then against the proteome of SARS-CoV by BLAST (version 2.2.25+). After that, Spike surface 20 glycoprotein (S) were picked, and RBD domain in Spike was also discriminated. For the protein sequence of Spike genes, we got, multiple sequence alignment was made by using the FFT-NS-2 algorithm in MAFFT v7.407 program, and three regions (50~246aa; author/funder. All rights reserved. No reuse allowed without permission.
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