Author: Rui Xiong; Leike Zhang; Shiliang Li; Yuan Sun; Minyi Ding; Yong Wang; Yongliang Zhao; Yan Wu; Weijuan Shang; Xiaming Jiang; Jiwei Shan; Zihao Shen; Yi Tong; Liuxin Xu; Chen Yu; Yingle Liu; Gang Zou; Dimitri Lavillete; Zhenjiang Zhao; Rui Wang; Lili Zhu; Gengfu Xiao; Ke Lan; Honglin Li; Ke Xu
Title: Novel and potent inhibitors targeting DHODH, a rate-limiting enzyme in de novo pyrimidine biosynthesis, are broad-spectrum antiviral against RNA viruses including newly emerged coronavirus SARS-CoV-2 Document date: 2020_3_12
ID: hq5um68k_39
Snippet: were treated with appropriate concentration of compounds in infection medium (DMEM+2% FBS+25 mM HEPES). After 7 days, the supernatant was collected and RNA was extracted by TRIzol Reagent (Invitrogen) and submitted to reverse transcription-quantitative PCR with the PrimeScript RT reagent kit (TaKaRa). The primers to detect Zika-specific RNA were listed below: Zika virus NS3 (F: 5'-CTCCAGGATGCAAGTCTAAG -3', R: 5'-ACCCAGCAGGAACTTCAGGA -3')......
Document: were treated with appropriate concentration of compounds in infection medium (DMEM+2% FBS+25 mM HEPES). After 7 days, the supernatant was collected and RNA was extracted by TRIzol Reagent (Invitrogen) and submitted to reverse transcription-quantitative PCR with the PrimeScript RT reagent kit (TaKaRa). The primers to detect Zika-specific RNA were listed below: Zika virus NS3 (F: 5'-CTCCAGGATGCAAGTCTAAG -3', R: 5'-ACCCAGCAGGAACTTCAGGA -3').
Search related documents:
Co phrase search for related documents- PrimeScript RT reagent kit and reagent kit: 1
- PrimeScript RT reagent kit and TaKaRa PrimeScript RT reagent kit: 1
- reagent kit and TaKaRa PrimeScript RT reagent kit: 1
- reagent kit and transcription quantitative pcr: 1, 2, 3
- reagent kit and transcription quantitative pcr reverse: 1, 2, 3
- transcription quantitative pcr and Zika virus: 1
- transcription quantitative pcr reverse and Zika virus: 1
Co phrase search for related documents, hyperlinks ordered by date