Selected article for: "false positive phenomenon and positive phenomenon"
Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2
  • Document date: 2020_2_27
    • ID: nbu6bsb3_37
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint (Figure5C and D) . Meanwhile, the differences of Ct values between neighbouring curves are approximately 3 cycles, which is very close to the theoretical 3.3 cycles f.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint (Figure5C and D) . Meanwhile, the differences of Ct values between neighbouring curves are approximately 3 cycles, which is very close to the theoretical 3.3 cycles for a 10-fold dilution. These results indicated that the RT-PCR amplification is highly efficient both in the presence or absence of pcMNPs. However, it is also important to note that in both cases, the negative controls sometimes gave observable amplification signals. Although their Ct values are lagged too far behind 40 cycles to be regarded as a valid positive result, this phenomenon raise concerns about possible false-positive issues, in which further optimization of primer pairs and probe might be necessary.

    Search related documents:
    Co phrase search for related documents
    • absence presence and Ct value: 1, 2, 3, 4, 5, 6, 7
    • absence presence and negative control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • absence presence and positive result: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • amplification signal and Ct value: 1, 2, 3, 4
    • amplification signal and dilution cycle: 1
    • amplification signal and negative control: 1
    • amplification signal and observable amplification signal: 1
    • amplification signal and primer pair: 1
    • Ct value and dilution cycle: 1, 2
    • Ct value and negative control: 1, 2, 3, 4, 5, 6, 7, 8, 9
    • Ct value and positive result: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • Ct value and RT PCR amplification: 1, 2, 3, 4, 5, 6, 7
    • negative control and positive result: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23
    • negative control and primer pair: 1, 2
    • negative control and probe primer pair: 1
    • negative control and RT PCR amplification: 1, 2
    • positive result and RT PCR amplification: 1, 2, 3, 4, 5, 6, 7, 8
    • positive result and valid positive result: 1
    • primer pair and probe primer pair: 1, 2, 3, 4