Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA Document date: 2020_3_29
ID: nil1vv6h_14
Snippet: Of the 11 water filtrate samples tested, two samples from Mongolia (WM3 and WM20) (18.2%) had viral contigs with 100% identity to the Equid herpesvirus 1 and 3 (EHV-1 and EHV-3). The contig of WM20 mapped to the membrane glycoprotein B, whereas the two contigs of WM3 to the DNA packaging protein and membrane glycoprotein G, all regions covered by the Herpesviridae baits (Suppl. Fig. 10A -D). A nested panherpes PCR targeting the DNA polymerase gen.....
Document: Of the 11 water filtrate samples tested, two samples from Mongolia (WM3 and WM20) (18.2%) had viral contigs with 100% identity to the Equid herpesvirus 1 and 3 (EHV-1 and EHV-3). The contig of WM20 mapped to the membrane glycoprotein B, whereas the two contigs of WM3 to the DNA packaging protein and membrane glycoprotein G, all regions covered by the Herpesviridae baits (Suppl. Fig. 10A -D). A nested panherpes PCR targeting the DNA polymerase gene and the resulting Sanger sequences further confirmed EHV presence (Suppl. Fig. 10E ). Several equine species including domestic horses inhabit the Gobi Desert [9], which is consistent with the presence of these viruses.
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