Selected article for: "sequence variant and variant sequence"
Author: Felix Grünberger; Robert Knüppel; Michael Jüttner; Martin Fenk; Andreas Borst; Robert Reichelt; Winfried Hausner; Jörg Soppa; Sebastien Ferreira-Cerca; Dina Grohmann
Title: Nanopore-based native RNA sequencing provides insights into prokaryotic transcription, operon structures, rRNA maturation and modifications
  • Document date: 2019_12_19
    • ID: e5p4metz_43
    Snippet: Despite the high sequencing depth of the (pre-)rRNA, we did not detect a full-length precursor 540 consisting of the 16S leading-16S-tRNA-23S-23S trailing elements in P. furiosus and H. volcanii, 541 suggesting that early rRNA processing occurs rapidly in these cells. The remaining reads were 542 grouped according to their 5´ leading and 3´ trailing lengths into five additional categories that are 543 less abundant (with less than 1600 counts i.....
    Document: Despite the high sequencing depth of the (pre-)rRNA, we did not detect a full-length precursor 540 consisting of the 16S leading-16S-tRNA-23S-23S trailing elements in P. furiosus and H. volcanii, 541 suggesting that early rRNA processing occurs rapidly in these cells. The remaining reads were 542 grouped according to their 5´ leading and 3´ trailing lengths into five additional categories that are 543 less abundant (with less than 1600 counts in each group): (1) a processing variant that entails the 544 16S rRNA leading/trailing sequence-tRNA-23S rRNA leading/trailing sequence, (2) probably 545 fragmented full-length precursor rRNAs, (3) 16S rRNA leading/trailing sequence-tRNA, (4) 16S 546 rRNA leading/trailing sequence-tRNA-23S rRNA and (5) tRNA-23S rRNA (Fig. 5 , see 547 Supplementary Fig. 13 ). The early 16S rRNA leading/trailing sequence-tRNA-23S rRNA trailing 548 sequence precursor (1) or 16S rRNA leading/trailing sequence (3) generated by cleavage and 549 the author/funder. All rights reserved. No reuse allowed without permission.

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