Selected article for: "acid extraction and manual extraction"
Author: Mauricio J Farfan; Juan P Torres; Miguel ORyan; Mauricio Olivares; Pablo Gallardo; Carolina Salas
Title: Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing
  • Document date: 2020_4_17
    • ID: kuqinczz_14
    Snippet: To test the efficiency of our previous data, 20 new pools were prepared. Pools 13 to 26 included 4 negative samples and 1 positive sample with CT ranging from 16.6 to 36.1; pools 27 to 31 included 5 negative samples. Extraction of nucleic acid from pools were done by manual extraction. SARS-CoV-2 amplification was observed in pools 13 to 25, observing an increase of CT values from 1 to 4.5. No amplification signal was detected in pool 26, which i.....
    Document: To test the efficiency of our previous data, 20 new pools were prepared. Pools 13 to 26 included 4 negative samples and 1 positive sample with CT ranging from 16.6 to 36.1; pools 27 to 31 included 5 negative samples. Extraction of nucleic acid from pools were done by manual extraction. SARS-CoV-2 amplification was observed in pools 13 to 25, observing an increase of CT values from 1 to 4.5. No amplification signal was detected in pool 26, which include a positive sample with a CT=36.1. Pools 27 to 31 were all RT-PCR negative ( Figure 2 ).

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