Author: Mauricio J Farfan; Juan P Torres; Miguel ORyan; Mauricio Olivares; Pablo Gallardo; Carolina Salas
Title: Optimizing RT-PCR detection of SARS-CoV-2 for developing countries using pool testing Document date: 2020_4_17
ID: kuqinczz_9
Snippet: For RT-qPCR detection we used the TaqMan™ 2019-nCoV Assay Kit v1 for the Orf1ab gene, according to the manufacturer's instructions (ThermoFisher) in a LightCycler® 480 Instrument II (Roche), using 5 µl of nucleic acid extraction or pool samples. A cycle threshold (CT) <37 was considered positive......
Document: For RT-qPCR detection we used the TaqMan™ 2019-nCoV Assay Kit v1 for the Orf1ab gene, according to the manufacturer's instructions (ThermoFisher) in a LightCycler® 480 Instrument II (Roche), using 5 µl of nucleic acid extraction or pool samples. A cycle threshold (CT) <37 was considered positive.
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