Author: Stefania Marzinotto; Catia Mio; Adriana Cifu'; Roberto Verardo; Corrado Pipan; Claudio Schneider; Francesco Curcio
Title: A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA extraction Document date: 2020_4_11
ID: mi6w8ppe_21
Snippet: In conclusion, our treatment enables a SARS CoV-2 RNA amplification which is at least as sensitive and accurate as that performed with RNAs extracted using commercial kits. At this time of rapid spreading of COVID-19, the shortage of reagents for RNA extraction is significantly slowing down the testing process, which is the most effective way to contrast the pandemic. Our simple and fast protocol overcomes these limitations, bypassing the need fo.....
Document: In conclusion, our treatment enables a SARS CoV-2 RNA amplification which is at least as sensitive and accurate as that performed with RNAs extracted using commercial kits. At this time of rapid spreading of COVID-19, the shortage of reagents for RNA extraction is significantly slowing down the testing process, which is the most effective way to contrast the pandemic. Our simple and fast protocol overcomes these limitations, bypassing the need for RNA extraction reagents, shortening the time of the analysis and reducing its cost. 5-fold serial dilutions of a patient's sample isolated with the novel custom protocol (a-f). NTC was used to test sensitivity of both RT-qPCR (A) and RT-ddPCR (B).
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