Selected article for: "acid sample and loop primer primer"
Author: Deguo Wang
Title: One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay
  • Document date: 2020_4_22
    • ID: krim73zt_1
    Snippet: The 50-μL reaction mixture contained 0.8 mM each of forward inner primer (FIP) and backward inner primer (BIP), 0.2 mM each of forward outer primer (F3) and backward outer primer (B3), 0.4 mM of forward loop primer (LF) and backward loop primer (LB), 1.2 mM dNTPs, 1× Bst DNA Polymerase Buffer (Zhengzhou Shenxiang Industrial Co., Ltd, China), 0.1 mM MnSO 4 , 0.1 mM 4-(2-pyridylazo) resorcinol, 5 µL pork blood samples free of COVID-19, 10 µL 20.....
    Document: The 50-μL reaction mixture contained 0.8 mM each of forward inner primer (FIP) and backward inner primer (BIP), 0.2 mM each of forward outer primer (F3) and backward outer primer (B3), 0.4 mM of forward loop primer (LF) and backward loop primer (LB), 1.2 mM dNTPs, 1× Bst DNA Polymerase Buffer (Zhengzhou Shenxiang Industrial Co., Ltd, China), 0.1 mM MnSO 4 , 0.1 mM 4-(2-pyridylazo) resorcinol, 5 µL pork blood samples free of COVID-19, 10 µL 2019-nCoV-Fast-Sample Nucleic Acid Releasing Agent (Hunan Shengxiang Biology Technology Co., Ltd, China), 25 U Bst DNA/RNA Polymerase 3.0 (New England Biolabs, Inc., MA, USA), and pUC57-N DNA of 2-0.02 fg 9 .

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