Author: Monica Sentmanat; Evguenia Kouranova; Xiaoxia Cui
Title: One-step RNA extraction for RT-qPCR detection of 2019-nCoV Document date: 2020_4_5
ID: ihhu7nef_14
Snippet: Collection of a nasopharyngeal specimen (NP) is the recommended method for testing patients presenting COVID-19 symptoms using the CDC diagnostic RT-qPCR test, with oropharyngeal (OP) being an acceptable alternative according to CDC guidelines. To determine if direct lysis of comparable specimen samples could be used for the RT-qPCR test, we self-collected NP and OP samples using sterile polyester flock swabs (PurFlock Ultra flocked collection sw.....
Document: Collection of a nasopharyngeal specimen (NP) is the recommended method for testing patients presenting COVID-19 symptoms using the CDC diagnostic RT-qPCR test, with oropharyngeal (OP) being an acceptable alternative according to CDC guidelines. To determine if direct lysis of comparable specimen samples could be used for the RT-qPCR test, we self-collected NP and OP samples using sterile polyester flock swabs (PurFlock Ultra flocked collection swabs by Puritan Diagnostics LLC). The swabs were each placed in 1.5 mL Eppendorf tube containing 200 ul of QuickExtract DNA Extraction Solution (QE buffer, Lucigen LLC, Madison, WI). Each tube was vortexed and stored until extraction.
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