Author: Mandy Muller; Britt A. Glaunsinger
Title: Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases Document date: 2017_6_26
ID: jhwjh12k_38
Snippet: 3'UTR reporter along with a control empty vector or a plasmid expressing SOX. After 24 h, 821 total RNA was harvested and subjected to RT-qPCR to measure GFP mRNA levels. Protein 822 levels of HuR and NCL after siRNA-mediated depletion are shown under the bar graphs 823 (~30% and 45% of total protein left respectively) (C) 293T cells were transfected with the 824 GFP-GADD45B-3'UTR reporter along with a control empty vector or a plasmid expressing.....
Document: 3'UTR reporter along with a control empty vector or a plasmid expressing SOX. After 24 h, 821 total RNA was harvested and subjected to RT-qPCR to measure GFP mRNA levels. Protein 822 levels of HuR and NCL after siRNA-mediated depletion are shown under the bar graphs 823 (~30% and 45% of total protein left respectively) (C) 293T cells were transfected with the 824 GFP-GADD45B-3'UTR reporter along with a control empty vector or a plasmid expressing 825
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