Selected article for: "polymerase reaction and RNA template"
Author: Jingyue Ju; Xiaoxu Li; Shiv Kumar; Steffen Jockusch; Minchen Chien; Chuanjuan Tao; Irina Morozova; Sergey Kalachikov; Robert N. Kirchdoerfer; James J. Russo
Title: Nucleotide Analogues as Inhibitors of SARS-CoV Polymerase
  • Document date: 2020_3_14
    • ID: hj675z1b_8
    Snippet: These results demonstrate that lower fidelity polymerases will have a high likelihood of incorporating 2'-F,Me-UTP and inhibit viral RNA replication, whereas high fidelity enzymes, more typical of the host DNA and RNA polymerases, will have a low likelihood of being inhibited by 2'-F,Me-UTP. Anti-viral drug design based on this principle may lead to potent viral polymerase inhibitors with fewer side effects. To provide further proof that SARS-CoV.....
    Document: These results demonstrate that lower fidelity polymerases will have a high likelihood of incorporating 2'-F,Me-UTP and inhibit viral RNA replication, whereas high fidelity enzymes, more typical of the host DNA and RNA polymerases, will have a low likelihood of being inhibited by 2'-F,Me-UTP. Anti-viral drug design based on this principle may lead to potent viral polymerase inhibitors with fewer side effects. To provide further proof that SARS-CoV-2 RdRp might be inhibited by 2'-F,Me-UTP, we next tested the ability of this molecule to be incorporated into an RNA primer to terminate the reaction catalyzed by the RdRp from SARS-CoV, using an RNA template. As shown in Fig. 4a , the active triphosphate form of the drug not only was incorporated by the RdRp, but prevented further incorporation, behaving as a terminator in the polymerase reaction.

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