Selected article for: "appropriate reaction buffer and SARS CoV polymerase"
Author: Minchen Chien; Thomas K. Anderson; Steffen Jockusch; Chuanjuan Tao; Shiv Kumar; Xiaoxu Li; James J. Russo; Robert Kirchdoerfer; Jingyue Ju
Title: Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase
  • Document date: 2020_3_20
    • ID: 8prn86g6_8
    Snippet: terminate the polymerase reaction. The sequences of the primer and template used for these extension reactions, which are at the 3' end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2'-F,Me-UTP, (b) 3'-F-dTTP, (c) UTP + TFV-DP, and (d) 3'-N 3 -dTTP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8), the indicated RNA template and primer, and the appropria.....
    Document: terminate the polymerase reaction. The sequences of the primer and template used for these extension reactions, which are at the 3' end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2'-F,Me-UTP, (b) 3'-F-dTTP, (c) UTP + TFV-DP, and (d) 3'-N 3 -dTTP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8), the indicated RNA template and primer, and the appropriate reaction buffer, followed by detection of reaction products by MALDI-TOF MS. The detailed procedure is shown in the Methods section. The accuracy for m/z determination is ± 10 Da.

    Search related documents:
    Co phrase search for related documents
    • reaction buffer and SARS genome: 1, 2, 3, 4
    • reaction buffer and SARS genome end: 1
    • reaction buffer and SARS polymerase: 1, 2, 3
    • reaction buffer and template primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • reaction buffer and template primer sequence: 1, 2
    • reaction product and RNA template: 1
    • reaction product and SARS genome: 1
    • reaction product and template primer: 1, 2
    • reaction product detection and RNA template: 1
    • RNA template and SARS genome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
    • RNA template and SARS genome end: 1, 2
    • RNA template and SARS polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
    • RNA template and template primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43
    • RNA template and template primer sequence: 1, 2, 3, 4
    • SARS genome and template primer: 1, 2, 3, 4
    • SARS genome and template primer sequence: 1
    • SARS genome end and template primer: 1, 2
    • SARS polymerase and template primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
    • SARS polymerase and template primer sequence: 1, 2