Selected article for: "appropriate reaction buffer and UTP extension"
Author: Minchen Chien; Thomas K. Anderson; Steffen Jockusch; Chuanjuan Tao; Shiv Kumar; Xiaoxu Li; James J. Russo; Robert Kirchdoerfer; Jingyue Ju
Title: Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase
  • Document date: 2020_3_20
    • ID: 8prn86g6_8
    Snippet: terminate the polymerase reaction. The sequences of the primer and template used for these extension reactions, which are at the 3' end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2'-F,Me-UTP, (b) 3'-F-dTTP, (c) UTP + TFV-DP, and (d) 3'-N 3 -dTTP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8), the indicated RNA template and primer, and the appropria.....
    Document: terminate the polymerase reaction. The sequences of the primer and template used for these extension reactions, which are at the 3' end of the SARS-CoV-2 genome, are shown at the top of the figure. Polymerase extension reactions were performed by incubating (a) 2'-F,Me-UTP, (b) 3'-F-dTTP, (c) UTP + TFV-DP, and (d) 3'-N 3 -dTTP with pre-assembled SARS-CoV-2 polymerase (nsp12, nsp7 and nsp8), the indicated RNA template and primer, and the appropriate reaction buffer, followed by detection of reaction products by MALDI-TOF MS. The detailed procedure is shown in the Methods section. The accuracy for m/z determination is ± 10 Da.

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