Author: Darko Bosnakovski; Erik A. Toso; Olivia O. Recht; Anja Cucak; Abhinav K Jain; Michelle C. Barton; Michael Kyba
Title: p53 is not necessary for DUX4 pathology Document date: 2017_3_19
ID: jopeo9gs_26
Snippet: Grand Island, NY, 17101-015) and dispase (Gibco, 17105-041) . Single cell suspensions were plated in T75 dishes in F-10/Ham's (Hyclone) medium containing 20% FBS (HyClone), 50 ng μ L -1 human basic fibroblast growth factor (Peprotech), 1% penicillin/streptomycin (Gibco), and 1% Glutamax (Gibco) and cultured at 37°C at 5% O 2 , 5% CO 2 , for 5 days. Establishing overexpressing cell lines Retroviral expression constructs were co-transfected with .....
Document: Grand Island, NY, 17101-015) and dispase (Gibco, 17105-041) . Single cell suspensions were plated in T75 dishes in F-10/Ham's (Hyclone) medium containing 20% FBS (HyClone), 50 ng μ L -1 human basic fibroblast growth factor (Peprotech), 1% penicillin/streptomycin (Gibco), and 1% Glutamax (Gibco) and cultured at 37°C at 5% O 2 , 5% CO 2 , for 5 days. Establishing overexpressing cell lines Retroviral expression constructs were co-transfected with the packaging constructs pCL-Eco (Naviaux et al. 1996) into NIH-3T3 cells using FuGENE 6 transfection reagent (Roche). Viruscontaining supernatants were collected 48 hours later, filtered (0.45 μ m) and used directly to transduce iC2C12-DUX4 cells. Constitutively overexpressing cell lines were obtained by FACS sorting GFP+ cells. All rights reserved. No reuse allowed without permission.
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