Author: Darko Bosnakovski; Erik A. Toso; Olivia O. Recht; Anja Cucak; Abhinav K Jain; Michelle C. Barton; Michael Kyba
Title: p53 is not necessary for DUX4 pathology Document date: 2017_3_19
ID: jopeo9gs_27
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https: //doi.org/10.1101/118315 doi: bioRxiv preprint Cell death inhibition assays Cell viability was analyzed as previously described . Briefly, myoblasts and fibroblasts were plated in 96 well plates at 3000 cells per well in proliferation medium and treated with different concentrations of doxycycline for 72 h. Medium was removed from the plate; 100 µ.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https: //doi.org/10.1101/118315 doi: bioRxiv preprint Cell death inhibition assays Cell viability was analyzed as previously described . Briefly, myoblasts and fibroblasts were plated in 96 well plates at 3000 cells per well in proliferation medium and treated with different concentrations of doxycycline for 72 h. Medium was removed from the plate; 100 µL working (diluted 1:3 with PBS) CellTiter-Glo luminescent assay reagent (Promega) was added to each well and luminescence measured on a Cytation3 plate reader (BioTek). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/118315 doi: bioRxiv preprint exposed to doxycycline (dox) to induce DUX4 expression (below). Scale bar represents 100 μm.
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