Selected article for: "replication structure and virus replication"

Author: Lu, Jiaqi; Gao, Fei; Wei, Zuzhang; Liu, Ping; Liu, Changlong; Zheng, Haihong; Li, Yanhua; Lin, Tao; Yuan, Shishan
Title: A 5'-proximal Stem-loop Structure of 5' Untranslated Region of Porcine Reproductive and Respiratory Syndrome Virus Genome Is Key for Virus Replication
  • Cord-id: k4q6nx73
  • Document date: 2011_4_15
  • ID: k4q6nx73
    Snippet: BACKGROUND: It has been well documented that the 5' untranslated region (5' UTR) of many positive-stranded RNA viruses contain key cis-acting regulatory sequences, as well as high-order structural elements. Little is known for such regulatory elements controlling porcine arterivirus replication. We investigated the roles of a conserved stem-loop 2 (SL2) that resides in the 5'UTR of the genome of a type II porcine reproductive and respiratory syndrome virus (PRRSV). RESULTS: We provided genetic e
    Document: BACKGROUND: It has been well documented that the 5' untranslated region (5' UTR) of many positive-stranded RNA viruses contain key cis-acting regulatory sequences, as well as high-order structural elements. Little is known for such regulatory elements controlling porcine arterivirus replication. We investigated the roles of a conserved stem-loop 2 (SL2) that resides in the 5'UTR of the genome of a type II porcine reproductive and respiratory syndrome virus (PRRSV). RESULTS: We provided genetic evidences demonstrating that 1) the SL2 in type II PRRSV 5' UTR, N-SL2, could be structurally and functionally substituted by its counterpart in type I PRRSV, E-SL2; 2) the functionality of N-SL2 was dependent upon the G-C rich stem structure, while the ternary-loop size was irrelevant to RNA synthesis; 3) serial deletions showed that the stem integrity of N-SL2 was crucial for subgenomic mRNA synthesis; and 4) when extensive base-pairs in the stem region was deleted, an alternative N-SL2-like structure with different sequence was utilized for virus replication. CONCLUSION: Taken together, we concluded that the phylogenetically conserved SL2 in the 5' UTR was crucial for PRRSV virus replication, subgenomic mRNA synthesis in particular.

    Search related documents:
    Co phrase search for related documents
    • absence presence and long range: 1
    • absence presence and low efficiency: 1, 2
    • absence presence and low melting: 1
    • absence presence and low melting agarose: 1
    • absence presence and low sensitivity: 1, 2, 3, 4, 5, 6, 7
    • acute respiratory syndrome virus and low efficiency: 1
    • acute respiratory syndrome virus and low sensitivity: 1, 2, 3, 4, 5
    • long range and low efficiency: 1
    • long range and low sensitivity: 1