Author: Charles L Howe; Reghann G. LaFrance-Corey; Emma N Goddery; Kanish Mirchia
Title: Neuronal CCL2 expression drives inflammatory monocyte infiltration into the brain during acute virus infection Document date: 2017_10_25
ID: ebqquj7i_9
Snippet: RNA was isolated from the whole brain of mice following perfusion with ice cold PBS using the RNeasy Lipid Tissue Midi Kit (Qiagen, Valencia, CA). RNA integrity, purity, and concentration was assessed using an RNA Analysis kit (Agilent Technologies, Santa Clara, CA). Samples passing quality control were analyzed on Illumina mouse WG-6 v 2.0 expression BeadChips in the Mayo Clinic Medical Genome Facility Gene Expression Core. Expression data were .....
Document: RNA was isolated from the whole brain of mice following perfusion with ice cold PBS using the RNeasy Lipid Tissue Midi Kit (Qiagen, Valencia, CA). RNA integrity, purity, and concentration was assessed using an RNA Analysis kit (Agilent Technologies, Santa Clara, CA). Samples passing quality control were analyzed on Illumina mouse WG-6 v 2.0 expression BeadChips in the Mayo Clinic Medical Genome Facility Gene Expression Core. Expression data were analyzed using Excel and MATLAB, where fold change was calculated and converted to log 2 . Heatmaps and hierarchical clusters were derived using Gitools v2.2.2.
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