Novel ionophores active against La Crosse virus identified through rapid antiviral screening

Zachary J. Sandler; Michelle N. Vu; Vineet D. Menachery; Bryan C. Mounce

Transcription Kits (Thermo-Fischer), according to the manufacturer's protocol, with 10-100 ng of 333 RNA and random hexamer primers. 334 335 Viral genome quantification. Following cDNA synthesis, qRT-PCR was performed using the 336 QuantStudio3 (Applied Biosystems by Thermo-Fischer) and SYBR green mastermix 337 (DotScientific). Samples were held at 95⁰C for 2 mins prior to 40 cycles of 95⁰C for 1s and 60⁰C 338 for 30s. Primers were verified for linearity using eight-fold serial diluted cDNA and checked for 339 specificity via melt curve analysis following by agarose gel electrophoresis. All samples were used 340 to normalize to total RNA using the ΔC T method. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint replenishment. *p<0.05, **p<0.01, ***p<0.001 using two-tailed Student's T-test, n≥2. Error bars 502 represent one standard error of the mean. 503 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.21.914929 doi: bioRxiv preprint