Selected article for: "probe XFFSL primer and XFFSL primer"
Author: Dupas Enora; Briand Martial; Jacques Marie-Agnès; Cesbron Sophie
Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues
  • Document date: 2019_7_11
    • ID: 65qpcsiq_25
    Snippet: Primers and probes were designed within specific long-mers (Table 3) . Specific amplifications were 259 obtained in silico on XF genome sequences and WGS bacterial sequences from NCBI at the expected 260 amplification size, without any mismatch for the five primer and probe combinations (XFF, XFFSL, 261 XFM, XFMO and XFP). Only two mismatches were observed and concerned the XF primer and probe 262 combination. One mismatch was on the eighth nucle.....
    Document: Primers and probes were designed within specific long-mers (Table 3) . Specific amplifications were 259 obtained in silico on XF genome sequences and WGS bacterial sequences from NCBI at the expected 260 amplification size, without any mismatch for the five primer and probe combinations (XFF, XFFSL, 261 XFM, XFMO and XFP). Only two mismatches were observed and concerned the XF primer and probe 262 combination. One mismatch was on the eighth nucleotide on the XF probe for the Xfm Dixon, Griffin1, 263 M12, Sycamore, CFBP 8416, CFBP 8417, CFBP 8418 strains and the second one was on the sixth 264 nucleotide of the forward XF primer of the Ann-1 Xfs strain. As there were not many possible 265

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