Author: Dupas Enora; Briand Martial; Jacques Marie-Agnès; Cesbron Sophie
Title: Novel tetraplex qPCR assays for simultaneous detection and identification of Xylella fastidiosa subspecies in plant tissues Document date: 2019_7_11
ID: 65qpcsiq_48
Snippet: The use of Harper's primers and probe in set n°5 allowed the detection of Xf strain at the same LOD 352 than for XF primers and probe in spiked Q. robur samples, but the detection was slightly better (a gain 353 of one Log unit) in the spiked C. monspeliensis samples. A Ct value was obtained for all spiked samples 354 with the 18s rRNA primers, highlighting that these primers and probe were reliable internal 355 amplification controls. 356.....
Document: The use of Harper's primers and probe in set n°5 allowed the detection of Xf strain at the same LOD 352 than for XF primers and probe in spiked Q. robur samples, but the detection was slightly better (a gain 353 of one Log unit) in the spiked C. monspeliensis samples. A Ct value was obtained for all spiked samples 354 with the 18s rRNA primers, highlighting that these primers and probe were reliable internal 355 amplification controls. 356
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