Selected article for: "amplification efficiency and lamp reaction"
Author: Deguo Wang
Title: One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay
  • Document date: 2020_4_22
    • ID: krim73zt_5
    Snippet: Optimization of the real-time RT-LAMP reaction temperature For the RT-LAMP reaction, as shown in Figure 1 , all positive controls (with pUC57-N DNA as template) had amplification, and the results of all negative controls (DNA template substituted by ddH 2 O) were negative at 55°C, 57°C, 59°C and 91°C , however, there was no significant difference in the amplification efficiency, and 59°C was temporarily was selected for the subsequent experi.....
    Document: Optimization of the real-time RT-LAMP reaction temperature For the RT-LAMP reaction, as shown in Figure 1 , all positive controls (with pUC57-N DNA as template) had amplification, and the results of all negative controls (DNA template substituted by ddH 2 O) were negative at 55°C, 57°C, 59°C and 91°C , however, there was no significant difference in the amplification efficiency, and 59°C was temporarily was selected for the subsequent experiments.

    Search related documents:
    Co phrase search for related documents
    • amplification efficiency and negative control: 1, 2, 3
    • amplification efficiency and negative control result: 1
    • amplification efficiency and positive control: 1, 2, 3, 4, 5
    • amplification efficiency and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
    • amplification efficiency and RT LAMP reaction: 1
    • amplification efficiency and significant difference: 1
    • amplification efficiency significant difference and significant difference: 1
    • dna template and negative control: 1, 2, 3, 4
    • dna template and positive control: 1, 2, 3, 4, 5, 6, 7, 8
    • dna template and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
    • dna template and RT LAMP reaction: 1
    • dna template and template DNA positive control: 1, 2, 3, 4, 5, 6
    • negative control and positive control: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • negative control and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • negative control and significant difference: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
    • negative control and template DNA positive control: 1
    • negative control result and positive control: 1, 2
    • negative control result and real time: 1, 2
    • positive control and template DNA positive control: 1, 2, 3, 4, 5, 6, 7