Author: Deguo Wang
Title: One-pot Detection of COVID-19 with Real-time Reverse-transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assay and Visual RT-LAMP Assay Document date: 2020_4_22
ID: krim73zt_5
Snippet: Optimization of the real-time RT-LAMP reaction temperature For the RT-LAMP reaction, as shown in Figure 1 , all positive controls (with pUC57-N DNA as template) had amplification, and the results of all negative controls (DNA template substituted by ddH 2 O) were negative at 55°C, 57°C, 59°C and 91°C , however, there was no significant difference in the amplification efficiency, and 59°C was temporarily was selected for the subsequent experi.....
Document: Optimization of the real-time RT-LAMP reaction temperature For the RT-LAMP reaction, as shown in Figure 1 , all positive controls (with pUC57-N DNA as template) had amplification, and the results of all negative controls (DNA template substituted by ddH 2 O) were negative at 55°C, 57°C, 59°C and 91°C , however, there was no significant difference in the amplification efficiency, and 59°C was temporarily was selected for the subsequent experiments.
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