Selected article for: "bovine serum and cell assay"
Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair
  • Document date: 2020_3_25
    • ID: 4h930ksd_51
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.006270 doi: bioRxiv preprint Supplementary file 4 macrophages. Unattached cells were collected and centrifuged at 300 × g for 10 min at 4 o C. Cell pellets were then resuspended in a complete mouse medium (CMM: DMEM/F-12 supplemented with 2 mM L-glutamine, 0.25% bovine serum albumin, 10 mM HEPES, 0.1 mM non-essential amino acids, 0.05.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.24.006270 doi: bioRxiv preprint Supplementary file 4 macrophages. Unattached cells were collected and centrifuged at 300 × g for 10 min at 4 o C. Cell pellets were then resuspended in a complete mouse medium (CMM: DMEM/F-12 supplemented with 2 mM L-glutamine, 0.25% bovine serum albumin, 10 mM HEPES, 0.1 mM non-essential amino acids, 0.05% ITS, 100 µg/mL primocin, and 10% newborn calf serum). The viability of harvested AT2 cells was assessed by the trypan blue exclusion assay followed by cell counting for the yield.

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