Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair Document date: 2020_3_25
ID: 4h930ksd_63
Snippet: Transepithelial short-circuit current (ISC, μA/cm 2 ) in AT2 monolayers was measured with an 8-channel voltageclamp amplifier (Physiological instruments, USA) as previously described5. Briefly, AT2 monolayers were mounted in the vertical Ussing Chambers bathed with solutions containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.83 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, 10 HEPES, 10 mannitol (apical compartment), or 10 D-glucose (basolateral compartment).....
Document: Transepithelial short-circuit current (ISC, μA/cm 2 ) in AT2 monolayers was measured with an 8-channel voltageclamp amplifier (Physiological instruments, USA) as previously described5. Briefly, AT2 monolayers were mounted in the vertical Ussing Chambers bathed with solutions containing (in mM): 120 NaCl, 25 NaHCO3, 3.3 KH2PO4, 0.83 K2HPO4, 1.2 CaCl2, 1.2 MgCl2, 10 HEPES, 10 mannitol (apical compartment), or 10 D-glucose (basolateral compartment). Each solution was iso-osmotic. The transwell cultures were bubbled continuously with a gas mixture of 95% O2-5% CO2. The transmonolayer potential was short-circuited to 0 mV, and a 10-mV pulse author/funder. All rights reserved. No reuse allowed without permission.
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