Selected article for: "active triphosphate and RNA polymerase"
Author: Minchen Chien; Thomas K. Anderson; Steffen Jockusch; Chuanjuan Tao; Shiv Kumar; Xiaoxu Li; James J. Russo; Robert Kirchdoerfer; Jingyue Ju
Title: Nucleotide Analogues as Inhibitors of SARS-CoV-2 Polymerase
  • Document date: 2020_3_20
    • ID: 8prn86g6_4
    Snippet: Given the 98% amino acid similarity of the SARS-CoV and SARS-CoV-2 RdRps, 10 we reasoned that the four nucleotide analogues listed in Fig. 1 would also inhibit the SARS-CoV-2 polymerase. We thus assessed the ability of 2'-F,Me-UTP, 3'-F-dTTP, TFV-DP, and 3'-N 3 -dTTP (the active triphosphate forms of Sofosbuvir, Alovudine, TAF and AZT, respectively), to be incorporated by SARS-CoV-2 RdRp into an RNA primer and terminate the polymerase reaction......
    Document: Given the 98% amino acid similarity of the SARS-CoV and SARS-CoV-2 RdRps, 10 we reasoned that the four nucleotide analogues listed in Fig. 1 would also inhibit the SARS-CoV-2 polymerase. We thus assessed the ability of 2'-F,Me-UTP, 3'-F-dTTP, TFV-DP, and 3'-N 3 -dTTP (the active triphosphate forms of Sofosbuvir, Alovudine, TAF and AZT, respectively), to be incorporated by SARS-CoV-2 RdRp into an RNA primer and terminate the polymerase reaction.

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