Author: de Souza Luna, Luciano Kleber; Heiser, Volker; Regamey, Nicolas; Panning, Marcus; Drexler, Jan Felix; Mulangu, Sabue; Poon, Leo; Baumgarte, Sigrid; Haijema, Bert Jan; Kaiser, Laurent; Drosten, Christian
Title: Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray. Cord-id: wd8wers8 Document date: 2007_1_1
ID: wd8wers8
Snippet: A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
Document: A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
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