Selected article for: "actin anti and lysis buffer"
Author: Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan
Title: Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy
  • Document date: 2020_3_30
    • ID: 0l33i6s4_60
    Snippet: Cells were harvested and lysed in RIPA lysis buffer (Beyotime, P0013C) with a cocktail 5 of protease inhibitors (Biomake, B14001). Samples were applied to 10% or 12% SDS/PAGE 6 gels(BioRad) and transferred to PVDF membranes (millipore). Membranes were blocked with 5% 7 nonfat milk in PBS with 0.1% tween 20. Then probed with primary antibodies. The following 8 antibodies were used in this study: anti-MTHFD1 (Proteintech, 10794-1-AP), anti-beta act.....
    Document: Cells were harvested and lysed in RIPA lysis buffer (Beyotime, P0013C) with a cocktail 5 of protease inhibitors (Biomake, B14001). Samples were applied to 10% or 12% SDS/PAGE 6 gels(BioRad) and transferred to PVDF membranes (millipore). Membranes were blocked with 5% 7 nonfat milk in PBS with 0.1% tween 20. Then probed with primary antibodies. The following 8 antibodies were used in this study: anti-MTHFD1 (Proteintech, 10794-1-AP), anti-beta actin 9

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