Orthogonal genome-wide screenings in bat cells identify MTHFD1 as a target of broad antiviral therapy

Danielle E Anderson; Jin Cui; Qian Ye; Baoying Huang; Wenhong Zu; Jing Gong; Weiqiang Liu; So Young Kim; Biao Guo Yan; Kristmundur Sigmundsson; Xiao Fang Lim; Fei Ye; Peihua Niu; Xuming Zhou; Wenjie Tan; Lin-Fa Wang; Xu Tan

(Easybio, BE0022), anti PR8 M1 (Genetex, GTX125928-S), anti HA (H1N1) (Genetex, 10 P. alecto ACTIN forward primer: 5'-gccagtctacaccgtctgcag -3' 23 P. alecto ACTIN reverse primer: 5'-cgtaggaatccttctggcccatg-3' 24 P. alecto MTHFD1 forward primer: 5'-gggagcgactgaagaaccaag-3' 25 P. alecto MTHFD1 reverse primer: 5'-tcttcagcagccttcagcttcac-3' 26 P. alecto SEC23B forward primer: 5'-cagcgtttgaccaggaggcc-3' 27 P. alecto SEC23B reverse primer: 5'-gggtcagatcctgtcgggc -3' 28 P. alecto GAPDH forward primer: 5'-ATACTTCTCATGGTTCACAC -3' 29 P. alecto GAPDH reverse primer: 5'-TCATTGACCTCAACTACATG-3' 30 P. alecto ATP6V0D1 forward primer: 5'-GTGGTAGAGTTCCGCCACAT-3' 31 . CC-BY-NC 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.29.014209 doi: bioRxiv preprint P. alecto ATP6V0D1 reverse primer: 5'-CTCAAAGCTGCCTAGTGGGT-3' 1 PR8 M1 forward primer: 5'-TTCTAACCGAGGTCGAAACGTACG-3' 2 PR8 M1 reserve primer: 5'-ACAAAGCGTCTACGCTGCAG-3' 3 PR8 vRNA reserve transcription primer: 5'-AGCRAAAGCAGG-3' 4 ZIKV NS5 forward primer: 5'-GGTCAGCGTCCTCTCTAATAAACG-3' 5 ZIKV NS5 reserve primer: 5'-GCACCCTAGTGTCCACTTTTTCC-3' 6 7 Immunofluorescence 8