Author: Mehdi Baratchian; Jeff McManus; Mike Berk; Fumihiko Nakamura; Serpil Erzurum; Sanjay Mukhopadhyay; Judy Drazba; John Peterson; Ben Gaston; Nima Sharifi
Title: No evidence that androgen regulation of pulmonary TMPRSS2 explains sex-discordant COVID-19 outcomes Document date: 2020_4_21
ID: awb1stsr_34
Snippet: Approximately 40-50 mg freshly frozen lung was added to soft tissue homogenizing CK14 tubes (Betin Technologies) with 200 ml RIPA buffer containing HALT protease and phosphate inhibitor cocktail. Lung tissues were then homogenized with a homogenizer (Minilys, Betin Technologies) three times (60 s each time) at the highest speed, with 5-10 minute intervals on ice to cool lysates. The lysates were then centrifuged for 15 min at 16,000.....
Document: Approximately 40-50 mg freshly frozen lung was added to soft tissue homogenizing CK14 tubes (Betin Technologies) with 200 ml RIPA buffer containing HALT protease and phosphate inhibitor cocktail. Lung tissues were then homogenized with a homogenizer (Minilys, Betin Technologies) three times (60 s each time) at the highest speed, with 5-10 minute intervals on ice to cool lysates. The lysates were then centrifuged for 15 min at 16,000
Search related documents:
Co phrase search for related documents- high speed and lung tissue: 1
- high speed and soft tissue: 1, 2, 3, 4
- lung tissue and soft tissue: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
Co phrase search for related documents, hyperlinks ordered by date