No evidence that androgen regulation of pulmonary TMPRSS2 explains sex-discordant COVID-19 outcomes

Mehdi Baratchian; Jeff McManus; Mike Berk; Fumihiko Nakamura; Serpil Erzurum; Sanjay Mukhopadhyay; Judy Drazba; John Peterson; Ben Gaston; Nima Sharifi

The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.21.051201 doi: bioRxiv preprint never smoker; GSE994 = 34 current smoker/18 former smoker/23 never smoker. Data sets 18385, 63127, 7895, and 994 contained multiple TMPRSS2 reference sequences and the expression values of the different sequences were added together within each data set. In all of the data sets that contained four TMPRSS2 reference sequences (18385 both large and small airway and 63127), the difference between groups was largest for reference sequence AI660243, so expression using that reference sequence alone is also shown for those data sets. For data sets with two groups, p-values from t-tests are shown. For data sets with three groups, Tukey HSD p-values are shown that were obtained after one-way ANOVA (ANOVA expression and smoking pack years. Adjusted R-squared and p-values from linear fits for each data set: GSE16008 R 2 = 0.03 and p = 0.19; GSE18385 large airway R 2 = -0.03 and p = 0.76; GSE18385 small airway R 2 = -0.01 and p = 0.41; GSE37147 (current smokers) R 2 = -0.01 and p = 0.67; GSE37147 (former smokers) R 2 = 0.001 and p = 0.30; GSE7895 (current smokers) R 2 = 0.01 and p = 0.24; GSE7895 (former smokers) R 2 = -0.02 and p = 0.60. author/funder. All rights reserved. No reuse allowed without permission.