Nosocomial viral ventilator-associated pneumonia in the intensive care unit: a prospective cohort study

Daubin, Cédric; Vincent, Sophie; Vabret, Astrid; du Cheyron, Damien; Parienti, Jean-Jacques; Ramakers, Michel; Freymuth, François; Charbonneau, Pierre

OBJECTIVE: To determine the incidence, risk factors, and clinical relevance of viral ventilator-associated pneumonia (VAP) in an adult intensive care unit (ICU). DESIGN: Prospective observational study. SETTING: A 22-bed adult medical ICU in a university hospital. PATIENTS: All consecutive adult patients ventilated more than 48 h in a 9-month period including regular seasonal viral infections. INTERVENTIONS: A tracheobronchial aspirate upon enrollment and at the time of VAP suspicion. MEASUREMENTS AND RESULTS: All respiratory specimens were tested in culture, indirect immunofluorescence assay, and PCR or RT-PCR for virological assessment. Patients were followed until ICU discharge or death. One hundred thirty-nine patients were included. Upon enrollment, a respiratory virus was detected in the tracheobronchial aspirate in 25% of patients (35 of 139). The incidence of VAP, defined according to clinical daily evaluation, was 28% (39 of 139 patients). A bacteria was documented in 74% of cases, whereas no case of a causative viral infection was encountered among VAP patients; however, herpes simplex virus type-1 (HSV 1) infection was detected in respiratory specimens of 31% of VAP (12 of 39). CONCLUSIONS: We found a high incidence of HSV-1 infection in VAP patients; however, nosocomial viral VAP is likely to be rare in ICU, as assessed by the absence of respiratory virus-induced VAP identified in this prospective cohort study.