Selected article for: "cell line and gene transfer"
Author: João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad
Title: A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
  • Document date: 2018_12_26
    • ID: 1kugu5zk_24
    Snippet: The second part of the landing pad system is a transfer vector that encodes the synthetic gene circuit. In order to verify that the transfer vector is correctly integrated at the hAAVS1 landing pad (hAAVS1 LP), we positioned a promoter-less multicistronic cassette encoding Blasticidin resistance and nuclear localized tagBFP downstream of the BxBI attB site. When site specific integration is accomplished, the cell line switches color (from mRuby2 .....
    Document: The second part of the landing pad system is a transfer vector that encodes the synthetic gene circuit. In order to verify that the transfer vector is correctly integrated at the hAAVS1 landing pad (hAAVS1 LP), we positioned a promoter-less multicistronic cassette encoding Blasticidin resistance and nuclear localized tagBFP downstream of the BxBI attB site. When site specific integration is accomplished, the cell line switches color (from mRuby2 to tagBFP) and resistance (Hygromycin to Blasticidin). The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint

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