Evaluation of the influence of global DNA methylation level in patients with acute coronary syndrome.

Carla Silva Soares, Fábia; Amorim, Ester A S; Martins Araújo, Romário; Pereira Werkhauser, Roberto; Tadeu Nunes Diniz, George; Carvalho, Viviane Dcv; Silva, Lilían Ca; Montenegro, Sergio T; Silva, Lilían Ca; Montenegro, Sergio T; Moraes, Clarice N L; Martins, Danyelly B G; Montenegro, Sílvia M L

DNA methylation is one of the mechanisms of epigenetic regulation and is observed in mammals to maintain a normal expression pattern of the genes. Aberrant profiles of DNA methylation have already been associated with cardiovascular diseases. We evaluated 190 patients with Acute Coronary Syndrome (ACS) and 75 patients without ACS (non-ACS). Patient severity was assessed by the TIMI risk score, and both levels of global DNA methylation (ACS = 190; non-ACS = 75), stratified in expected group (male ≥ 65 years; female ≥ 55 years) and early group (male <65 years; female <55 years). As results, the ACS and non-ACS groups showed different levels of global DNA methylation, and patients with ACS were more methylated (p = 0.0121). Patients with ACS, showed a difference (p < 0.0001) in methylation profiles between groups. The low TIMI group had a higher level of DNA methylation, while the intermediate / high group showed a decreased methylation pattern. A negative correlation was observed between the level of global methylation and the increase in age (p = 0.0387; r = -0.15), which became hypomethylated over the years. The hypermethylated global DNA profile by its association with the development of ACS can be a potential biomarker.