A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells

João Pedro Fonseca; Alain R. Bonny; G. Renuka Kumar; Andrew H. Ng; Jason Town; Qiu Chang Wu; Elham Aslankoohi; Susan Y. Chen; Patrick Harrigan; Lindsey C. Osimiri; Amy L. Kistler; Hana El-Samad

A major asset of the MTK is a large, characterized suite of modular parts to build versatile TUs. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint modification of the GG protocol (See "end-on" GG Protocol, Methods). In addition, while the MTK allows flexibility in the assembly of a wide variety of TUs, the sequence of TUs in a final multi-TU plasmid is prescribed at the TU assembly step (e.g. a TU assembled with an LS part 1 will always be the most upstream TU). Therefore, it is prudent to follow general design principles in order to maximize their repurposing 33 The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/506188 doi: bioRxiv preprint ZEBOV-4cis cell lines also exhibit susceptibility to previously identified chemical inhibitors. Thus, the MTK provides a potentially powerful tool for virologists to scale functional experimental studies apace with the recent explosive growth in viral genome sequences 34, 35 .