Alignment of multiple metabolomics LC-MS datasets from disparate diseases to reveal fever-associated metabolites

Năstase, Ana-Maria; Barrett, Michael P.; Cárdenas, Washington B.; Cordeiro, Fernanda Bertuccez; Zambrano, Mildred; Andrade, Joyce; Chang, Juan; Regato, Mary; Carrillo, Eugenia; Botana, Laura; Moreno, Javier; Milne, Kathryn; Spence, Philip J.; Rowe, J. Alexandra; Rogers, Simon

Acute febrile illnesses are still a major cause of mortality and morbidity globally, particularly in low to middle income countries. The aim of this study was to determine any possible metabolic commonalities of patients infected with disparate pathogens that cause fever. Three liquid chromatography-mass spectrometry (LC-MS) datasets investigating the metabolic effects of malaria, leishmaniasis and Zika virus infection were used. The retention time (RT) drift between the datasets was determined using landmarks obtained from the standard reference mixtures generally used in the quality control of the LC-MS experiments. We used fitted Gaussian Process models (GPs) to perform a high level correction of the RT drift between the experiments, followed by standard peakset alignment between the samples with corrected RTs of the three LC-MS datasets. Statistical analysis, annotation and pathway analysis of the integrated peaksets were subsequently performed. Metabolic dysregulation patterns common across the datasets were identified, with kynurenine pathway being the most affected pathway between all three fever-associated datasets.