Author: Ulanova, T I; Puzyrev, V F; Riabinina, S A; Burkov, A N; Obriadina, A P
Title: [Theoretical prediction of the antigenic epitopes of severe acute respiratory syndrome (SARS-CoV) proteins and evaluation of their diagnostic value]. Cord-id: mllzgvdq Document date: 2005_1_1
ID: mllzgvdq
Snippet: Analysis of the sequence of a number of proteins of the virus causing severe acute respiratory syndrome (SARS-CoV) was used to theoretically predict antigenic epitopes. Nine sequences, encoding for the predicted epitopes in spike, nucleocapside, and membranous SARS CoV proteins, were synthesized by polymyrase chain reaction and cloned into the pGEX 4-T2 vector. The resultant plasmids were expressed in the E. coil cells and purified by glutathione-Sepharose affinity chromatography.
Document: Analysis of the sequence of a number of proteins of the virus causing severe acute respiratory syndrome (SARS-CoV) was used to theoretically predict antigenic epitopes. Nine sequences, encoding for the predicted epitopes in spike, nucleocapside, and membranous SARS CoV proteins, were synthesized by polymyrase chain reaction and cloned into the pGEX 4-T2 vector. The resultant plasmids were expressed in the E. coil cells and purified by glutathione-Sepharose affinity chromatography.
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