Selected article for: "death rate and mean death rate"
Author: Liu, Zhao; Wei, Fei; Chen, Liang-Jun; Xiong, Hai-Rong; Liu, Yuan-Yuan; Luo, Fan; Hou, Wei; Xiao, Hong; Yang, Zhan-Qiu
Title: In Vitro and in Vivo Studies of the Inhibitory Effects of Emodin Isolated from Polygonum cuspidatum on Coxsakievirus B(4)
  • Cord-id: jfihkob6
  • Document date: 2013_9_25
    • ID: jfihkob6
    Snippet: The lack of effective therapeutics for Coxsackievirus B(4) (CVB(4)) infection underscores the importance of finding novel antiviral compounds. Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is one of the natural anthraquinone derivatives obtained from the root and rhizome of Polygonum cuspidatum. In the present study, the possibility of using emodin as a potential antiviral to treat CVB(4) infection was explored in vitro and in mice. Emodin reduced CVB(4) entry and replication on Hep-2 cells in
    Document: The lack of effective therapeutics for Coxsackievirus B(4) (CVB(4)) infection underscores the importance of finding novel antiviral compounds. Emodin (1,3,8-trihydroxy-6-methylanthraquinone) is one of the natural anthraquinone derivatives obtained from the root and rhizome of Polygonum cuspidatum. In the present study, the possibility of using emodin as a potential antiviral to treat CVB(4) infection was explored in vitro and in mice. Emodin reduced CVB(4) entry and replication on Hep-2 cells in a concentration- and time-dependent manner, with a 50% effective concentration (EC(50)) of 12.06 μM and selectivity index (SI) of 5.08, respectively. The inhibitory effect of emodin for CVB(4) entry and replication was further confirmed by a quantitative real time PCR (qPCR) assay. The results further showed that the mice orally treated with different dosages of emodin displayed a dose dependent increase of survival rate, body weight and prolonged mean time of death (MTD), accompanied by significantly decreased myocardial virus titers and pathologic scores/lesions. Moreover, emodin could inhibit CVB(4)-induced apoptosis in vitro and in vivo. Our results indicated that emodin could be used as potential antiviral in the post-exposure prophylaxis for CVB(4) infection.

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