Author: Niccolo Alfano; Anisha Dayaram; Jan Axtner; Kyriakos Tsangaras; Marie-Louise Kampmann; Azlan Mohamed; Seth Timothy Wong; M. Thomas P. Gilbert; Andreas Wilting; Alex Daivd Greenwood
Title: Non-invasive surveys of mammalian viruses using environmental DNA Document date: 2020_3_29
ID: nil1vv6h_20
Snippet: Using short RNA baits to capture highly conserved sequences from every known vertebrate viral genome is a useful and relatively inexpensive approach for providing an initial viral identification. However, to fully characterize each virus, the RNA oligonucleotide bait set would need to be customized to retrieve full length viral genomes. Initial screening with full length genomes for all viruses is costly and may result in detection of host DNA in.....
Document: Using short RNA baits to capture highly conserved sequences from every known vertebrate viral genome is a useful and relatively inexpensive approach for providing an initial viral identification. However, to fully characterize each virus, the RNA oligonucleotide bait set would need to be customized to retrieve full length viral genomes. Initial screening with full length genomes for all viruses is costly and may result in detection of host DNA in cases of spurious homology between viruses and host DNA sequence.
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